2D Heteronuclear Overhauser Effect SpectroscopY (HOESY) is an effective way to determine whether or not a pair of heteronuclear spins are close to one another in space. It is particularly effective for 1H and 31P where both nuclides are 100% naturally abundant. 2D experiments, however, can be quite time consuming. Alternatively, one can obtain 1D 1H detected 1H - 31P HOESY data to save data collection time. When only one 31P resonance is present, the data can be obtained using nonselective 31P pulses. An example of this, using the, using the pulse sequence from the reference1 below, is shown in the figure. The HOESY spectrum is on top while the simple 1H spectrum is on the bottom. One can see that heteronuclear 1H - 31P NOE's are apparent on the bridging methylene protons and the ortho-aromatic protons. Neither the meta- nor para-aromatic protons show significant heteronuclear NOE's.
1. L.E. Combettes, P. Clausen-Thue, M.A. King, B. Odell, A.L. Thompson, V. Gouverneur and T.D.W. Claridge. Chem. Eur. J. 18, 13133 (2012).