Friday, September 12, 2008
Baseline Correction in 2D NMR Spectra
Sometimes a 1D NMR spectrum will have a baseline roll. One way of correcting this is to fit the baseline of the spectrum (between two limits) to a polynomial and then subtract the polynomial from the spectrum to produce a flat baseline. Baseline roll is not an exclusive problem to 1D data. It can also be present in each domain of a 2D data set. Baseline orrection can be applied to each of the dimensions. The figure below shows a proton NOESY spectrum which is uncorrected (top left), baseline corrected in the rows (top right), baseline corrected in the columns (bottom left) and baseline corrected in both domains (bottom right).