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Tuesday, November 6, 2007

19F - 1H HOESY Experiment

Many of you use proton NOESY spectra to establish whether or not two types of protons are close to one another in space. The same type of experiment is also possible between nuclei of different isotopes. This technique is called HOESY (Heteronuclear Overhauser Effect SpectroscopY) and can be used to assess the spatial proximity between 2 heteronuclei. Below is an example of a 19F detected 19F - 1H HOESY spectrum of a sugar. (sample courtesy of Jennifer Chaytor)

10 comments:

Anonymous said...

What pulse sequence do you use?

Glenn Facey said...

I used a slightly modified version of the one given in the Bruker pulse program library.

Anonymous said...

Is fhis HOESY experiment published and wher?
What is the modification of the standard HOESY bruker pulse program?
Thank you
bernard.ancian@upmc.fr

Glenn Facey said...

Bernard,

These data have not been published. The modification I made to the hoesyfhqfqnrv program was minor. I can't recall at the moment exactly what the modification was except that it was a minor change needed to run a newer pulse program on an older instrument.

Glenn

Narendra Reddy said...

Is it possible to record 1H-27Al HOESY experiment?
If possible please suggest me HOESY pulse sequence in Bruker spectrometer.

Glenn Facey said...

Narendra,

Thank you for your question. In principle a 1H - 27Al HOESY is possible however the T2 of the Al is likely to be too short to permit a hereronuclear NOE measurement. What is the line width of the 27Al resonance?

If the 27Al T2 is sufficiently long, you could try the "hoesyph" pulse program.

Glenn

Narendra Reddy said...

Thanks for your valuable response.
line width of 27Al resonance is 3389Hz in 500MHz spectrometer.

Glenn Facey said...

Narendra,

In the case of a 3.8 kkHz 27Al resonance, I think that a 1H-27Al HOESY measurement is not practical.

Glenn

Anonymous said...

I need to run 1H{19F} HOESY since the 19F molecule has a very high T1. Do you have the pulse sequence for the inverse HOESY?

Glenn Facey said...

Anonymous,

I have tried collecting 1H detected 2D 1H-19F HOESY data however my results were full of artifacts. I really am not sure why this was the case but I think it had something to do with my hardware. I do not have a working pulse sequence for you. I would start by modifying the hoesyfhqfqnrv pulse program in the Bruker library. Good luck.

Glenn